Neuronal activity in motor neuron and V2a interneuron cultures

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dc.contributor.advisorSakiyama-Elbert, Shelly E.
dc.contributor.committeeMemberWang, Huiliang (Evan)
dc.creatorSomavarapu, Rachel
dc.creator.orcid0000-0001-6016-4021
dc.date.accessioned2023-11-07T00:39:06Z
dc.date.available2023-11-07T00:39:06Z
dc.date.created2022-08
dc.date.issued2022-08-03
dc.date.submittedAugust 2022
dc.date.updated2023-11-07T00:39:07Z
dc.description.abstractThe consequences of a spinal cord injury can include paralysis, and loss of motor function, sensory function, and proprioception. Furthermore, spinal cord injuries are often associated with potentially fatal complications, such as pneumonia or sepsis. Spinal cord injuries can take a negative toll on a patient’s life by causing discomfort or by hindering them from living a normal life. Current treatments, such as rehabilitative therapy, electrical spinal cord stimulation, and spinal cord surgery are not effective in treating this condition. However, studying the behavior of cultured neurons in-vitro can help determine what combination of cells are most beneficial for spinal cord transplantation or targeting regeneration of endogenous neural populations. The goal of this project is to study two types of neurons that are found in the spinal cord, motor neurons and V2a interneurons, and examine how these cells behave when they are cultured separately versus cocultured together. Selectable cell lines containing puromycin N-acetyl-transferase (PAC) under the control of either the Hb9 or Chx10 gene regulatory element were induced and selected to form neurons over a 6 day period. The neurons were plated in multielectrode array (MEA) plates or 24 well plates. Collected data regarding the neural firing rate, burst frequency, number of spikes observed, network inter-spike interval coefficient of variation, and synchrony were analyzed to determine differences in activity between mono- and co-cultures.
dc.description.departmentBiomedical Engineering
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/2152/122478
dc.identifier.urihttp://dx.doi.org/10.26153/tsw/49282
dc.language.isoen
dc.subjectMotor neuron
dc.subjectV2a interneuron
dc.subjectInterneuron
dc.subjectSpinal cord
dc.subjectSpinal cord injury
dc.subjectNeuronal culture
dc.titleNeuronal activity in motor neuron and V2a interneuron cultures
dc.typeThesis
dc.type.materialtext
local.embargo.lift2024-08-01
local.embargo.terms2024-08-01
thesis.degree.departmentBiomedical Engineering
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorThe University of Texas at Austin
thesis.degree.levelMasters
thesis.degree.nameMaster of Science in Engineering

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