Effect of CSLM imaging rate on biofilms of P. aeruginosa and S. aureus

Simple item page
dc.contributor.advisorGordon, Vernita
dc.creatorDu, Reginald
dc.date.accessioned2017-05-10T15:44:44Z
dc.date.available2017-05-10T15:44:44Z
dc.date.issued2015-05
dc.description.abstractBiofilms are sessile communities of bacteria that can be found in an wide range of environments. Their inhabitants are phenotypically distinct from plank- tonic bacteria and are capable of forming complex, three-dimensional structures. Biofilms are studied using confocal scanning laser microscopy, or CSLM. This technique uses lasers and Novel Fluorescent Proteins (NFPs) to measure growth and structure formation of single- and multi-species biofilms in situ in three dimensions. We investigate the effects of slow and fast rates of image acquisition on mono- and co-cultures of biofilm forming bacteria: Pseudomonas aeruginosa and Staphylococcus aureus. After calculating growth rates and lag times, we find that fast scanning rates reduce the growth rate of P. aeruginosa in co-culture. Additionally, co-culture speeds up P. aeruginosa growth relative to monoculture when imaged at a slow rate, and fast scanning reverts co-culture growth to monoculture-like behavior. Additionally, a significant lag time is observed for P. aeruginosa grown in co-culture. The observed influence of confocal imaging rate on population dynamics should be considered in future studies to ensure accurate measurement of bacterial phenomena.en_US
dc.description.departmentPhysicsen_US
dc.identifierdoi:10.15781/T24X54N53
dc.identifier.urihttp://hdl.handle.net/2152/46797
dc.language.isoengen_US
dc.relation.ispartofHonors Thesesen_US
dc.rights.restrictionOpenen_US
dc.subjectbiofilmsen_US
dc.subjectconfocalen_US
dc.subjectphysicsen_US
dc.subjectmicrobiologyen_US
dc.subjectbacteriaen_US
dc.subjectpseudomonasen_US
dc.subjectstaphylococcusen_US
dc.subjectfadeden_US
dc.titleEffect of CSLM imaging rate on biofilms of P. aeruginosa and S. aureusen_US
dc.typeThesisen_US

Access full-text files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
ReggieFinalThesisMay2016.pdf
Size:
2.6 MB
Format:
Adobe Portable Document Format
Description:
Download

License bundle

Now showing 1 - 2 of 2
No Thumbnail Available
Name:
license.txt
Size:
1.66 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
No Thumbnail Available
Name:
Reggie_Du_Consent_form_May2016.pdf
Size:
208.83 KB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Honors Theses