Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors
| dc.creator | Zhang, Zichao | en |
| dc.creator | Wlodarczyk, Bogdan J. | en |
| dc.creator | Niederreither, Karen | en |
| dc.creator | Venugopalan, Shankar | en |
| dc.creator | Florez, Sergio | en |
| dc.creator | Finnell, Richard H. | en |
| dc.creator | Amendt, Brad A. | en |
| dc.date.accessioned | 2013-05-06T16:29:24Z | en |
| dc.date.available | 2013-05-06T16:29:24Z | en |
| dc.date.issued | 2011-09-14 | en |
| dc.description | Zichao Zhang is with Texas A&M Health Science Center; Shankar Venugopalan is with Texas A&M Health Science Center; Sergio Florez is with Texas A&M Health Science Center; Brad A. Amendt is with Texas A&M Health Science Center; Bogdan J. Wlodarczyk is with UT Austin; Karen Niederreither is with UT Austin; Richard H. Finnell is with UT Austin. | en |
| dc.description.abstract | The planar cell polarity effector gene Fuz regulates ciliogenesis and Fuz loss of function studies reveal an array of embryonic phenotypes. However, cilia defects can affect many signaling pathways and, in humans, cilia defects underlie several craniofacial anomalies. To address this, we analyzed the craniofacial phenotype and signaling responses of the Fuz−/− mice. We demonstrate a unique role for Fuz in regulating both Hedgehog (Hh) and Wnt/β-catenin signaling during craniofacial development. Fuz expression first appears in the dorsal tissues and later in ventral tissues and craniofacial regions during embryonic development coincident with cilia development. The Fuz−/− mice exhibit severe craniofacial deformities including anophthalmia, agenesis of the tongue and incisors, a hypoplastic mandible, cleft palate, ossification/skeletal defects and hyperplastic malformed Meckel's cartilage. Hh signaling is down-regulated in the Fuz null mice, while canonical Wnt signaling is up-regulated revealing the antagonistic relationship of these two pathways. Meckel's cartilage is expanded in the Fuz−/− mice due to increased cell proliferation associated with the up-regulation of Wnt canonical target genes and decreased non-canonical pathway genes. Interestingly, cilia development was decreased in the mandible mesenchyme of Fuz null mice, suggesting that cilia may antagonize Wnt signaling in this tissue. Furthermore, expression of Fuz decreased expression of Wnt pathway genes as well as a Wnt-dependent reporter. Finally, chromatin IP experiments demonstrate that β-catenin/TCF-binding directly regulates Fuz expression. These data demonstrate a new model for coordination of Hh and Wnt signaling and reveal a Fuz-dependent negative feedback loop controlling Wnt/β-catenin signaling. | en |
| dc.description.department | Dell Pediatric Research Institute | en |
| dc.description.sponsorship | This work was supported by the National Institutes of Health grants DE016315 to RHF, DE13941 and DE18885 to BAA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en |
| dc.identifier.citation | Zhang Z, Wlodarczyk BJ, Niederreither K, Venugopalan S, Florez S, et al. (2011) Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors. PLoS ONE 6(9): e24608. doi:10.1371/journal.pone.0024608 | en |
| dc.identifier.doi | 10.1371/journal.pone.0024608 | en |
| dc.identifier.uri | http://hdl.handle.net/2152/20096 | en |
| dc.language.iso | eng | en |
| dc.publisher | Public Library of Science | en |
| dc.rights | Attribution 3.0 United States | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | en |
| dc.subject | Beta-catenin signaling | en |
| dc.subject | Cartilage | en |
| dc.subject | Cilia | en |
| dc.subject | Embryos | en |
| dc.subject | Gene expression | en |
| dc.subject | Hedgehog signaling | en |
| dc.subject | Mandible | en |
| dc.subject | Wnt signaling cascade | en |
| dc.title | Fuz Regulates Craniofacial Development through Tissue Specific Responses to Signaling Factors | en |
| dc.type | Article | en |