Show simple item record

dc.creatorMcCaul, Kate Christina
dc.date.accessioned2012-07-18T21:08:02Z
dc.date.available2012-07-18T21:08:02Z
dc.date.created2012-05
dc.date.issued2012-07-18
dc.date.submittedMay 2012
dc.identifier.urihttp://hdl.handle.net/2152/ETD-UT-2012-05-5601
dc.descriptiontext
dc.description.abstractBurkholderia pseudomallei and Burkholderia mallei cause the diseases melioidosis and glanders, respectively. These diseases are endemic mainly in southeastern Asia and northern Australia, but they also pose a bioterrorism threat in the developed world. These diseases have high mortality, partially due to the lack of vaccines and rapid, accurate diagnostic assays. The work discussed here represents a part of a larger project to develop a dependable diagnostic assay for use in both developing endemic areas and the developed world, as well as a subunit vaccine to protect against disease. In this study, several proteins from B. pseudomallei, B. mallei, and the closely related but less virulent B. thailandensis have been cloned, expressed and purified in order to develop highly sensitive and specific diagnostic reagents for the detection of B. pseudomallei and B. mallei in infected patient samples. Protein targets expressed in this study were also used in subunit vaccine development for melioidosis and glanders.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.subjectBurkholderia pseudomallei
dc.subjectBurkholderia mallei
dc.subjectDiagnostics
dc.subjectVaccine
dc.subjectProtein
dc.subjectExpression
dc.subjectCloning
dc.subjectPurification
dc.titleCloning, expression, and purification of Burkholderia protein targets for diagnostic and vaccine development
dc.date.updated2012-07-18T21:08:49Z
dc.identifier.slug2152/ETD-UT-2012-05-5601
dc.description.departmentCellular and Molecular Biology
dc.type.genrethesis*
thesis.degree.departmentCellular and Molecular Biology
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorUniversity of Texas at Austin
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record