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dc.contributor.advisorBrown, Katherine A.en
dc.contributor.advisorKitto, George B.en
dc.creatorMcCaul, Kate Christinaen
dc.date.accessioned2012-07-18T21:08:02Zen
dc.date.available2012-07-18T21:08:02Zen
dc.date.created2012-05en
dc.date.issued2012-07-18en
dc.date.submittedMay 2012en
dc.identifier.urihttp://hdl.handle.net/2152/ETD-UT-2012-05-5601en
dc.descriptiontexten
dc.description.abstractBurkholderia pseudomallei and Burkholderia mallei cause the diseases melioidosis and glanders, respectively. These diseases are endemic mainly in southeastern Asia and northern Australia, but they also pose a bioterrorism threat in the developed world. These diseases have high mortality, partially due to the lack of vaccines and rapid, accurate diagnostic assays. The work discussed here represents a part of a larger project to develop a dependable diagnostic assay for use in both developing endemic areas and the developed world, as well as a subunit vaccine to protect against disease. In this study, several proteins from B. pseudomallei, B. mallei, and the closely related but less virulent B. thailandensis have been cloned, expressed and purified in order to develop highly sensitive and specific diagnostic reagents for the detection of B. pseudomallei and B. mallei in infected patient samples. Protein targets expressed in this study were also used in subunit vaccine development for melioidosis and glanders.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.subjectBurkholderia pseudomalleien
dc.subjectBurkholderia malleien
dc.subjectDiagnosticsen
dc.subjectVaccineen
dc.subjectProteinen
dc.subjectExpressionen
dc.subjectCloningen
dc.subjectPurificationen
dc.titleCloning, expression, and purification of Burkholderia protein targets for diagnostic and vaccine developmenten
dc.date.updated2012-07-18T21:08:49Zen
dc.identifier.slug2152/ETD-UT-2012-05-5601en
dc.description.departmentCellular and Molecular Biologyen
dc.type.genrethesisen
thesis.degree.departmentCellular and Molecular Biologyen
thesis.degree.disciplineCell and Molecular Biologyen
thesis.degree.grantorUniversity of Texas at Austinen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Artsen


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