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dc.contributor.advisorShubeita, George T.en
dc.creatorHolzmeister, Phil Jacken
dc.date.accessioned2011-11-02T19:18:38Zen
dc.date.available2011-11-02T19:18:38Zen
dc.date.issued2010-08en
dc.date.submittedAugust 2010en
dc.identifier.urihttp://hdl.handle.net/2152/ETD-UT-2010-08-1923en
dc.descriptiontexten
dc.description.abstractMolecular motors are responsible for all long range transport and organization of organelles within cells. However, little is known about the interaction of multiple similar and dissimilar motors. In this thesis I describe experiments to probe the coordination of the motors kinesin and dynein which move towards the opposite ends of microtubules. Cargos they haul show bidirectional movement at short scales yet there is net transport in one direction or the other. Two distinct models for the bidirectional transport exist: regulation and a tug-of-war. In order to differentiate between them, kinesin-specific antibodies are injected into Drosophila embryos and the effect on transport of lipid droplets is quantified and compared to unperturbed motion. The function-blocking antibodies resulted in an increased run length of dynein-mediated transport and a decrease in that of kinesin. Furthermore, reduced velocities in both directions and a trend towards shorter pauses were observed. Comparison of these results to predictions the models provide for this scenario supports a tug-of-war model rather than regulation.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.subjectMolecular motorsen
dc.subjectKinesinen
dc.subjectDyneinen
dc.subjectTug-of-waren
dc.titleProbing the coupling mechanism of opposite polarity motorsen
dc.date.updated2011-11-02T19:19:07Zen
dc.identifier.slug2152/ETD-UT-2010-08-1923en
dc.contributor.committeeMemberFlorin, Ernst-Ludwigen
dc.description.departmentPhysicsen
dc.type.genrethesisen
thesis.degree.departmentPhysicsen
thesis.degree.disciplinePhysicsen
thesis.degree.grantorUniversity of Texas at Austinen
thesis.degree.levelMastersen
thesis.degree.nameMaster of Artsen


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