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    Developing a diagnostic tool for acyl carrier proteins through trypsinolysis, reverse-phase chromatography and native chemical ligation

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    REYES-MASTERS-REPORT.pdf (519.0Kb)
    Date
    2010-08
    Author
    Reyes, Graciela, 1957-
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    Abstract
    Polyketide biosynthesis is a field that has had tremendous advances in the past 50 years. The understanding of the mechanisms is updated as investigations delve into domain interactions of these microbial natural products. Although numerous polyketides are known, similarities in the sequence of product generation can be used as templates for further exploration of enzymatic activity. The focus of studies recently has been towards developing protocols to manipulate the natural products resulting in medicinally important manufactured products. This investigation examined the mechanism of the acyl carrier protein (ACP) module involved in biosynthesis.
    Department
    Science and Mathematics Education
    Description
    text
    Subject
    ACP
    Product generation
    Enzymatic activity
    Biosynthesis
    Acyl carrier protein
    URI
    http://hdl.handle.net/2152/ETD-UT-2010-08-1725
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