• Alcohol Disinhibition of Behaviors in C. elegans 

      Topper, Stephen M.; Aguilar, Sara C.; Topper, Viktoria Y.; Elbel, Erin; Pierce-Shimomura, Jonathan T. (PLOS One, 2014-03-28)
      Alcohol has a wide variety of effects on physiology and behavior. One of the most well-recognized behavioral effects is disinhibition, where behaviors that are normally suppressed are displayed following intoxication. A ...
    • Alcohol-Induced Histone Acetylation Reveals a Gene Network Involved in Alcohol Tolerance 

      Ghezzi, Alfredo; Krishnan, Harish R.; Lew, Linda; Prado III, Francisco J.; Ong, Darryl S.; Atkinson, Nigel S. (PLOS Genetics, 2013-12-12)
      Sustained or repeated exposure to sedating drugs, such as alcohol, triggers homeostatic adaptations in the brain that lead to the development of drug tolerance and dependence. These adaptations involve long-term changes ...
    • Chronic self-administration of alcohol results in elevated ΔFosB: comparison of hybrid mice with distinct drinking patterns 

      Ozburn, Angela R.; Mayfield, RD; Ponomarev, Igor; Jones, Theresa A.; Blednov, Yuri A.; Harris, RA (BMC Neuroscience, 2012-10-29)
      Background: The inability to reduce or regulate alcohol intake is a hallmark symptom for alcohol use disorders. Research on novel behavioral and genetic models of experience-induced changes in drinking will further our ...
    • A DNA Element Regulates Drug Tolerance and Withdrawal in Drosophila 

      Li, Xiaolei; Ghezzi, Alfredo; Pohl, Jascha B.; Bohm, Arun Y.; Atkinson, Nigel S. (PLOS One, 2013-09-23)
      Drug tolerance and withdrawal are insidious responses to drugs of abuse; the first increases drug consumption while the second punishes abstention. Drosophila generate functional tolerance to benzyl alcohol sedation by ...
    • GABAA Receptors Containing ρ1 Subunits Contribute to In Vivo Effects of Ethanol in Mice 

      Blednov, Yuri A.; Benavidez, Jillian M.; Black, Mendy; Leiter, Courtney R.; Osterndorff-Kahanek, Elizabeth; Johnson, David; Borghese, Cecilia M.; Hanrahan, Jane R.; Johnston, Graham A.R.; Chebib, Mary; Harris, R. Adron (PLOS One, 2014-01-16)
      GABAA receptors consisting of ρ1, ρ2, or ρ3 subunits in homo- or hetero-pentamers have been studied mainly in retina but are detected in many brain regions. Receptors formed from ρ1 are inhibited by low ethanol concentrations, ...
    • Genome-wide analysis of transposable elements and tandem repeats in the compact placozoan genome 

      Wnag, Shi; Zhang, Lingling; Meyer, Eli; Bao, Shenmin (Biology Direct, 2010-04-15)
      The placozoan Trichoplax adhaerens has a compact genome with many primitive eumetazoan characteristics. In order to gain a better understanding of its genome architecture, we conducted a detailed analysis of repeat content ...
    • Integration of miRNA and Protein Profiling Reveals Coordinated Neuroadaptations in the Alcohol-Dependent Mouse Brain 

      Gorini, Giorgio; Nunez. Yuury O.; Mayfield, R. Dayne (PLOS One, 2013-12-16)
      The molecular mechanisms underlying alcohol dependence involve different neurochemical systems and are brain region-dependent. Chronic Intermittent Ethanol (CIE) procedure, combined with a Two-Bottle Choice voluntary ...
    • Interchangeable Punishments During Aversive Conditioning in Drosophila 

      Robinson, Brooks G.; Khurana, Sukant; Nguyen, Andrew; Chuang, Tammy; Young, Melvin; Yu, Jun; Hooten, Jeff; Atkinson, Nigel S. (2013-11)
      Using Drosophila melanogaster larvae we asked whether distinct aversive stimuli have a common neural representation during associative learning. We tested the interchangeability of heat shock and electroshock punishments ...
    • Molecular Mechanism for the Dual Alcohol Modulation of Cys-loop Receptors 

      Murail, Samuel; Howard, Rebecca J.; Broemstrup, Torben; Bertaccini, Edward J.; Harris, R. Adron; Trudell, James R.; Lindahl, Erik (Public Library of Science, 2012-10-04)
      Cys-loop receptors constitute a superfamily of pentameric ligand-gated ion channels (pLGICs), including receptors for acetylcholine, serotonin, glycine and γ-aminobutyric acid. Several bacterial homologues have been ...
    • Neuroadaptations in Human Chronic Alcoholics: Dysregulation of the NF-κB System 

      Ökvist, Anna; Johansson, Sofia; Kuzmin, Alexander; Bazov, Igor; Merino-Martinez, Roxana; Ponomarev, Igor; Mayfield, R. Dayne; Harris, R. Adron; Sheedy, Donna; Garrick, Therese; Harper, Clive; Hurd, Yasmin L.; Terenius, Lars; Ekström, Tomas J.; Bakalkin, Georgy; Yakovleva, Tatjana (Public Library of Science, 2007-09-26)
      Background -- Alcohol dependence and associated cognitive impairments apparently result from neuroadaptations to chronic alcohol consumption involving changes in expression of multiple genes. Here we investigated whether ...
    • Neurobiological Signatures of Alcohol Dependence Revealed by Protein Profiling 

      Gorini, Giorgio; Roberts, Amanda J.; Mayfield, Dayne R. (PLOS One, 2013-12-16)
      Alcohol abuse causes dramatic neuroadaptations in the brain, which contribute to tolerance, dependence, and behavioral modifications. Previous proteomic studies in human alcoholics and animal models have identified candidate ...
    • Positively Correlated miRNA-miRNA Regulatory Networks in Mouse Frontal Cortex During Early Stages of Alcohol Dependence 

      Nunez, Yury O.; Truitt, Jay M.; Gorini, Giorgio; Ponomareva, Olga N.; Blednov, Yuri A.; Harris, R. Adon; Mayfield, R. Dayne (2013-10)
      Although the study of gene regulation via the action of specific microRNAs (miRNAs) has experienced a boom in recent years, the analysis of genome-wide interaction networks among miRNAs and respective targeted mRNAs has ...
    • Positively correlated miRNA-mRNA regulatory networks in mouse frontal cortex during early stages of alcohol dependence 

      Nunez, Yury O.; Truitt, Jay M.; Gorini, Giorgio; Ponomareva, Olga N.; Blendnov; Harris, R. Adron; Mayfield, R. Dayne (BMC Genomics, 2013-10-22)
      Background: Although the study of gene regulation via the action of specific microRNAs (miRNAs) has experienced a boom in recent years, the analysis of genome-wide interaction networks among miRNAs and respective targeted ...
    • RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction 

      Lee, Changhoon; Harris, R. Adron; Wall, Jason K.; Mayfield, R. Dayne; Wilke, Claus O. (Biology Direct, 2013-07-04)
      Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. ...
    • RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction 

      Lee, Changhoon; Harris, R. Adron; Wall, Jason K.; Mayfield, R. Dayne; Wilke, Claus O. (2013-07)
      Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. ...