Iyer Laboratory: Gel-free Library Preparation Protocol
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Originally developed by Amelia Weber Hall in October 2012, then modified to avoid usage of silica gel columns to improve yield. Used for preparing libraries of DNA derived from chromatin immunoprecipitation experiments by the Iyer lab, and distributed to several other labs across Central Texas. Documented by Amelia Weber Hall, December 2012, March 2015, and June 2017. This protocol should work well with any DNA that needs to be prepared into a library for high-throughput sequencing, however it is highly optimized for small (nanogram to pictogram) quantities of DNA.
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