• Login
    • Submit
    View Item 
    •   Repository Home
    • UT Electronic Theses and Dissertations
    • UT Electronic Theses and Dissertations
    • View Item
    • Repository Home
    • UT Electronic Theses and Dissertations
    • UT Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The regulation of immunoglobulin gene expression

    Icon
    View/Open
    Access restricted to UT Austin EID holders (1.263Mb)
    Date
    2007-12
    Author
    Luo, Hong, 1980-
    Share
     Facebook
     Twitter
     LinkedIn
    Metadata
    Show full item record
    Abstract
    Antibody is diversified during B cell development to achieve high affinity to encountered antigens and multiple effecter functions. Somatic hypermutation (SHM), gene conversion (GC) and class switch recombination (CSR) are involved in diversification process. Activation induced cytidine deaminase (AID) is a B cell specific protein that initiates all three distinctive reactions by introducing cytosine to uracil mutation in immunoglobulin (Ig) genes. The mutagenic process is largely confined to specific regions of the Ig loci to prevent genomic instability. The targeting mechanism is unclear. To address this question, we used chicken B cell line DT40 as a working system to characterize the cis-acting elements and trans-activating factors involved in the control machinery. DT40 cells undergo gene conversion at the Ig loci. A major advantage of the system is that homologous recombination is efficient in this cell line. This property permits genetic analyses. Taking advantage of this property, we have made a series of deletions on the endogenous locus of Ig lambda (Igl) light chain gene. Based on the phenotypes of these mutations, we have defined the regions that control the expression of Igl. Moreover, these deletions revealed aberrant Igl transcripts. We are currently analyzing the effects of these mutations on gene conversion activity. In a complementary approach, we are knocking out the genes that may encode trans-acting factors for Igl gene expression and gene conversion. We have generated complete knockouts of interferon regulatory factor 4 (IRF4) and knocked out one allele of PU.1 gene. The targeted cell lines will be analyzed for Igl transcription and gene conversion.
    Department
    Microbiology
    Subject
    Immunoglobulin (Ig) genes
    B cell development
    Gene conversion (GC)
    URI
    http://hdl.handle.net/2152/46583
    Collections
    • UT Electronic Theses and Dissertations
    University of Texas at Austin Libraries
    • facebook
    • twitter
    • instagram
    • youtube
    • CONTACT US
    • MAPS & DIRECTIONS
    • JOB OPPORTUNITIES
    • UT Austin Home
    • Emergency Information
    • Site Policies
    • Web Accessibility Policy
    • Web Privacy Policy
    • Adobe Reader
    Subscribe to our NewsletterGive to the Libraries

    © The University of Texas at Austin

    Browse

    Entire RepositoryCommunities & CollectionsDate IssuedAuthorsTitlesSubjectsDepartmentThis CollectionDate IssuedAuthorsTitlesSubjectsDepartment

    My Account

    Login

    Information

    AboutContactPoliciesGetting StartedGlossaryHelpFAQs

    Statistics

    View Usage Statistics
    University of Texas at Austin Libraries
    • facebook
    • twitter
    • instagram
    • youtube
    • CONTACT US
    • MAPS & DIRECTIONS
    • JOB OPPORTUNITIES
    • UT Austin Home
    • Emergency Information
    • Site Policies
    • Web Accessibility Policy
    • Web Privacy Policy
    • Adobe Reader
    Subscribe to our NewsletterGive to the Libraries

    © The University of Texas at Austin