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dc.contributor.advisorShear, Jason B.
dc.creatorLu, Zhou, 1978-
dc.date.accessioned2017-04-26T14:11:01Z
dc.date.available2017-04-26T14:11:01Z
dc.date.issued2007-12
dc.identifierdoi:10.15781/T2736M71M
dc.identifier.urihttp://hdl.handle.net/2152/46582
dc.description.abstractConcentration of lactate in human blood or saliva is an important indicator for cardiac diseases. Conventional measurement of analytes in complex solution usually requires separation, which is time consuming. To bypass these problems, we developed enzyme based sensor arrays that rapidly and simultaneously analyze multiple analytes in solutions without separation. In our approach, porous agarose beads, with enzymes for L-lactate immobilized on them, were localized to wells etched into a silicon chip in a 5 + 7 array. Analysis was accomplished by measuring fluorescence signal difference generated by enzymes catalyzed reactions. In this thesis, I reported the development of a sensitive (detection limit [similar to] 5[Greek small letter mu]M) and rapid (within minutes) lactate assay using double-enzyme based sensor array. Lactate concentration in healthy people at rest was not detected partially due to enzyme inhibitions by protein adsorptions. Measurement of enzyme activity on individual beads is another research performed in this thesis. Success in this study should provide a more precise control over site-specific cellular manipulation by delivering a particle, decorated with optimal amount of effectors, into specific location of a living cell using optical trapping. In our approach, alkaline phosphatase (ALP) was used as an effector to generate product emitting fluorescence signal. Enzyme activity on individual microsphere was evaluated by signal strength. Separation of a single or small number of beads was accomplished using methods based on microfabrication via multiphoton excitation. The fluorescence signal was not detected on single beads partially due to the enzyme inhibition due to BSA quenching.en_US
dc.format.mediumelectronicen_US
dc.language.isoengen_US
dc.relation.ispartofUT Electronic Theses and Dissertationsen_US
dc.rightsCopyright © is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en_US
dc.subjectLactateen_US
dc.subjectHuman blooden_US
dc.subjectHuman salivaen_US
dc.subjectCardiac diseaseen_US
dc.subjectMeasurementen_US
dc.titleEnzyme microspheres for biosensing and cell signaling perturbationen_US
dc.typeThesisen_US
dc.description.departmentCellular and Molecular Biologyen_US
dc.type.genreThesisen_US
thesis.degree.departmentCellular and Molecular Biologyen_US
thesis.degree.disciplineCellular and Molecular Biologyen_US
thesis.degree.grantorUniversity of Texas at Austinen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
dc.rights.restrictionRestricteden_US


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