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dc.creatorTraeger, Lindsay L.en_US
dc.creatorVolkening, Jeremy D.en_US
dc.creatorMoffett, Howellen_US
dc.creatorGallant, Jason R.en_US
dc.creatorChen, Po-Haoen_US
dc.creatorNovina, Carl D.en_US
dc.creatorPhillips, George N., Jr.en_US
dc.creatorAnand, Reneen_US
dc.creatorWells, Gregg B.en_US
dc.creatorPinch, Matthewen_US
dc.creatorGueth, Roberten_US
dc.creatorUnguez, Graciela A.en_US
dc.creatorAlbert, James S.en_US
dc.creatorZakon, Harolden_US
dc.creatorSussman, Michael R.en_US
dc.creatorSamanta, Manoj P.en_US
dc.date.accessioned2016-10-28T19:53:31Z
dc.date.available2016-10-28T19:53:31Z
dc.date.issued2015-03en_US
dc.identifierdoi:10.15781/T2ST7F07W
dc.identifier.citationTraeger, Lindsay L., Jeremy D. Volkening, Howell Moffett, Jason R. Gallant, Po-Hao Chen, Carl D. Novina, George N. Phillips et al. "Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus)." BMC genomics, Vol. 16, No. 1 (Mar., 2015): 243.en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://hdl.handle.net/2152/43348
dc.description.abstractWith its unique ability to produce high-voltage electric discharges in excess of 600 volts, the South American strong voltage electric eel (Electrophorus electricus) has played an important role in the history of science. Remarkably little is understood about the molecular nature of its electric organs. Results: We present an in-depth analysis of the genome of E. electricus, including the transcriptomes of eight mature tissues: brain, spinal cord, kidney, heart, skeletal muscle, Sachs' electric organ, main electric organ, and Hunter's electric organ. A gene set enrichment analysis based on gene ontology reveals enriched functions in all three electric organs related to transmembrane transport, androgen binding, and signaling. This study also represents the first analysis of miRNA in electric fish. It identified a number of miRNAs displaying electric organ-specific expression patterns, including one novel miRNA highly over-expressed in all three electric organs of E. electricus. All three electric organ tissues also express three conserved miRNAs that have been reported to inhibit muscle development in mammals, suggesting that miRNA-dependent regulation of gene expression might play an important role in specifying an electric organ identity from its muscle precursor. These miRNA data were supported using another complete miRNA profile from muscle and electric organ tissues of a second gymnotiform species. Conclusions: Our work on the E. electricus genome and eight tissue-specific gene expression profiles will greatly facilitate future research on determining the coding and regulatory sequences that specify the function, development, and evolution of electric organs. Moreover, these data and future studies will be informed by the first comprehensive analysis of miRNA expression in an electric fish presented here.en_US
dc.description.sponsorshipNSF 1144012, 0741450, 1248109en_US
dc.description.sponsorshipW.M. Keck Foundation Distinguished Young Scholars in Medical Researchen_US
dc.description.sponsorshipNIH R01 GM084879, R01 GM088670, 1SC1GM092297-01A1en_US
dc.description.sponsorshipMorgridge Graduate Fellowshipen_US
dc.description.sponsorshipUniversity of Wisconsin Genetics NIH Graduate Training Granten_US
dc.description.sponsorshipCornell University Center for Vertebrate Genomicsen_US
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.subjectelectric eelen_US
dc.subjectgenomeen_US
dc.subjecttranscriptomeen_US
dc.subjectmirnaen_US
dc.subjectgene ontologyen_US
dc.subjectskeletal-muscleen_US
dc.subjectdifferential expressionen_US
dc.subjectcolor-visionen_US
dc.subjectmicrornasen_US
dc.subjectevolutionen_US
dc.subjectorganen_US
dc.subjectgenesen_US
dc.subjectcontributesen_US
dc.subjectcomplexityen_US
dc.subjectzebrafishen_US
dc.subjectbiotechnology & applied microbiologyen_US
dc.subjectgenetics & heredityen_US
dc.titleUnique Patterns of Transcript and miRNA Expression in the South American Strong Voltage Electric Eel (Electrophorus Electricus)en_US
dc.typeArticleen_US
dc.description.departmentIntegrative Biologyen_US
dc.rights.restrictionOpenen_US
dc.identifier.doi10.1186/s12864-015-1288-8en_US
dc.contributor.utaustinauthorZakon, Harolen_US
dc.relation.ispartofserialBMC Genomicsen_US


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