Show simple item record

dc.creatorHarcombe, W. R.en_US
dc.creatorSpringman, R.en_US
dc.creatorBull, J. J.en_US
dc.date.accessioned2016-10-28T19:50:11Z
dc.date.available2016-10-28T19:50:11Z
dc.date.issued2009-05en_US
dc.identifierdoi:10.15781/T27S7HV9K
dc.identifier.citationHarcombe, W. R., R. Springman, and J. J. Bull. "Compensatory evolution for a gene deletion is not limited to its immediate functional network." BMC evolutionary biology, Vol. 9, No. 1 (May, 2009): 1.en_US
dc.identifier.issn1471-2148en_US
dc.identifier.urihttp://hdl.handle.net/2152/43182
dc.description.abstractGenetic disruption of an important phenotype should favor compensatory mutations that restore the phenotype. If the genetic basis of the phenotype is modular, with a network of interacting genes whose functions are specific to that phenotype, compensatory mutations are expected among the genes of the affected network. This perspective was tested in the bacteriophage T3 using a genome deleted of its DNA ligase gene, disrupting DNA metabolism. Results: In two replicate, long-term adaptations, phage compensatory evolution accommodated the low ligase level provided by the host without reinventing its own ligase. In both lines, fitness increased substantially but remained well below that of the intact genome. Each line accumulated over a dozen compensating mutations during long-term adaptation, and as expected, many of the compensatory changes were within the DNA metabolism network. However, several compensatory changes were outside the network and defy any role in DNA metabolism or biochemical connection to the disruption. In one line, these extra-network changes were essential to the recovery. The genes experiencing compensatory changes were moderately conserved between T3 and its relative T7 (25% diverged), but the involvement of extra-network changes was greater in T3. Conclusion: Compensatory evolution was only partly limited to the known functionally interacting partners of the deleted gene. Thus gene interactions contributing to fitness were more extensive than suggested by the functional properties currently ascribed to the genes. Compensatory evolution offers an easy method of discovering genome interactions among specific elements that does not rest on an a priori knowledge of those elements or their interactions.en_US
dc.description.sponsorshipen_US
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.subjectcomplete nucleotide-sequenceen_US
dc.subjectescherichia-colien_US
dc.subjectbacteriophage t7en_US
dc.subjectreceptor specificityen_US
dc.subjectbeta-galactosidaseen_US
dc.subjecthost-rangeen_US
dc.subjectdnaen_US
dc.subjectmachineryen_US
dc.subjectphenotypeen_US
dc.subjectfitnessen_US
dc.subjectevolutionary biologyen_US
dc.subjectgenetics & heredityen_US
dc.titleCompensatory Evolution for a Gene Deletion is Not Limited to its Immediate Functional Networken_US
dc.typeArticleen_US
dc.description.departmentIntegrative Biologyen_US
dc.rights.restrictionOpenen_US
dc.identifier.doi10.1186/1471-2148-9-106en_US
dc.contributor.utaustinauthorHarcombe, W. R.en_US
dc.contributor.utaustinauthorSpringman, R.en_US
dc.contributor.utaustinauthorBull, J. J.en_US
dc.relation.ispartofserialBMC Evolutionary Biologyen_US


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record