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dc.creatorMeyer, Elien_US
dc.creatorAspinwall, Michael J.en_US
dc.creatorLowry, David B.en_US
dc.creatorPalacio-Mejia, Juan Diegoen_US
dc.creatorLogan, Tierney L.en_US
dc.creatorFay, Phillip A.en_US
dc.creatorJuenger, Thomas E.en_US
dc.date.accessioned2016-10-28T19:49:40Z
dc.date.available2016-10-28T19:49:40Z
dc.date.issued2014-06en_US
dc.identifierdoi:10.15781/T2BN9X56F
dc.identifier.citationMeyer, Eli, Michael J. Aspinwall, David B. Lowry, Juan Diego Palacio-Mejía, Tierney L. Logan, Philip A. Fay, and Thomas E. Juenger. "Integrating transcriptional, metabolomic, and physiological responses to drought stress and recovery in switchgrass (Panicum virgatum L.)." BMC genomics, Vol. 15, No. 1 (Jun., 2014): 1.en_US
dc.identifier.issn1471-2164en_US
dc.identifier.urihttp://hdl.handle.net/2152/43158
dc.description.abstractIn light of the changes in precipitation and soil water availability expected with climate change, understanding the mechanisms underlying plant responses to water deficit is essential. Toward that end we have conducted an integrative analysis of responses to drought stress in the perennial C-4 grass and biofuel crop, Panicum virgatum (switchgrass). Responses to soil drying and re-watering were measured at transcriptional, physiological, and metabolomic levels. To assess the interaction of soil moisture with diel light: dark cycles, we profiled gene expression in drought and control treatments under pre-dawn and mid-day conditions. Results: Soil drying resulted in reduced leaf water potential, gas exchange, and chlorophyll fluorescence along with differential expression of a large fraction of the transcriptome (37%). Many transcripts responded differently depending on time of day (e.g. up-regulation pre-dawn and down-regulation mid-day). Genes associated with C-4 photosynthesis were down-regulated during drought, while C-4 metabolic intermediates accumulated. Rapid changes in gene expression were observed during recovery from drought, along with increased water use efficiency and chlorophyll fluorescence. Conclusions: Our findings demonstrate that drought responsive gene expression depends strongly on time of day and that gene expression is extensively modified during the first few hours of drought recovery. Analysis of covariation in gene expression, metabolite abundance, and physiology among plants revealed non-linear relationships that suggest critical thresholds in drought stress responses. Future studies may benefit from evaluating these thresholds among diverse accessions of switchgrass and other C-4 grasses.en_US
dc.description.sponsorshipNational Science Foundation Plant Genome Research Program Award IOS-0922457en_US
dc.description.sponsorshipUnited States Department of Agriculture NIFA-AFRI postdoctoral fellowship 2011-67012-30696en_US
dc.description.sponsorshipHawkesbury Institute for the Environment, University of Western Sydneyen_US
dc.language.isoEnglishen_US
dc.relation.ispartofen_US
dc.rightsAdministrative deposit of works to Texas ScholarWorks: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access or the publisher allows a PDF version of the article to be freely posted online. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en_US
dc.subjectdroughten_US
dc.subjectrecoveryen_US
dc.subjectswitchgrassen_US
dc.subjectpanicum virgatumen_US
dc.subjectgene expressionen_US
dc.subjectrna-seqen_US
dc.subjectgene-expression atlasen_US
dc.subjectwater-stressen_US
dc.subjectarabidopsis-thalianaen_US
dc.subjectplant-responsesen_US
dc.subjectclimate-changeen_US
dc.subjectwhole-planten_US
dc.subjectcold stressen_US
dc.subjectdata setsen_US
dc.subjectphotosynthesisen_US
dc.subjectgenomicsen_US
dc.subjectbiotechnology & applied microbiologyen_US
dc.subjectgenetics & heredityen_US
dc.titleIntegrating Transcriptional, Metabolomic, and Physiological Responses to Drought Stress and Recovery in Switchgrass (Panicum Virgatum L.)en_US
dc.typeArticleen_US
dc.description.departmentIntegrative Biologyen_US
dc.rights.restrictionOpenen_US
dc.identifier.doi10.1186/1471-2164-15-527en_US
dc.contributor.utaustinauthorMeyer, Elien_US
dc.contributor.utaustinauthorAspinwall, Michael J.en_US
dc.contributor.utaustinauthorLowry, David B.en_US
dc.contributor.utaustinauthorPalacio-Mejia, Juan Diegoen_US
dc.contributor.utaustinauthorLogan, Tierney L.en_US
dc.contributor.utaustinauthorJuenger, Thomas E.en_US
dc.relation.ispartofserialBMC Genomicsen_US


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