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    Reaction-Based Indicator Displacement Assay (RIA) for the Selective Colorimetric and Fluorometric Detection of Peroxynitrite

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    Date
    2015-03
    Author
    Sun, Xiaolong
    Lacina, Karel
    Ramsamy, Elena C.
    Flower, Stephen E.
    Fossey, John S.
    Qian, Xuhong
    Anslyn, Eric V.
    Bull, Steven D.
    James, Tony D.
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    Abstract
    Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N, N-dimethylaminomethyl) phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS-NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B-N interaction. Our simple system produces a visible colorimetric change and on-off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reaction-based Indicator displacement Assay).
    Department
    Chemistry
    Subject
    appended bipyridinium salts
    boronic acid
    hydrogen-peroxide
    nitric-oxide
    fluorescent-probes
    sensing system
    glucose
    chemosensor
    reporter
    viologen
    chemistry, multidisciplinary
    URI
    http://hdl.handle.net/2152/41178
    Citation
    Sun, Xiaolong, Karel Lacina, Elena C. Ramsamy, Stephen E. Flower, John S. Fossey, Xuhong Qian, Eric V. Anslyn, Steven D. Bull, and Tony D. James. "Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite." Chemical Science 6, no. 5 (Mar., 2015): 2963-2967.
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