dc.contributor.advisor | Rimer, Mendell | en |
dc.contributor.advisor | Thompson, Wesley J. | en |
dc.creator | Ellerton, Elaine Louise | en |
dc.date.accessioned | 2008-08-29T00:25:14Z | en |
dc.date.available | 2008-08-29T00:25:14Z | en |
dc.date.issued | 2008-05 | en |
dc.identifier | b70710284 | en |
dc.identifier.uri | http://hdl.handle.net/2152/4003 | en |
dc.description | text | en |
dc.description.abstract | Schwann cell (SCs) are the glia of the peripheral nervous system. During
development, a common precursor develops into two distinct types of SCs: myelinating
SCs and non-myelinating SCs. There is little literature regarding the non-myelinating
variety of SCs, specifically a type of non-myelinating SC found at the neuromuscular
junction (NMJ), the terminal Schwann cell (tSC). Terminal SCs are critical for the
maintenance and recovery of the NMJ. Peripheral nerve injury causes tSCs to become
reactive, a state characterized by changes in gene expression and the extension of cellular
processes. It is in this state, that tSCs help to restore functionality to the denervated
junction. What drives tSCs to become reactive after injury remains largely unknown.
Previously, nine zinc-finger proteins (ZFPs), a class of transcription factors, have been
implicated in SC development and differentiation. As a result, transcription factors from
the ZFP family were considered as potential candidates that may drive the activation of
tSCs. Because tSCs are few and far between, only two-six cells cover the NMJ, I used a
largely non-myelinated nerve of the autonomic system, the cervical sympathetic trunk
(CST), to search for ZFP candidates. I created a cDNA library from both control and
denervated CST resulting in 40 unique ZFPs. Six of these genes were studied futher:
Zipro1, Zfp36, Zfp612, Zfp180, Zfp111 and Zfp629. I found a near two-fold increase in
Zipro1 mRNA and protein in denervated CST, and no change in the other five ZFPs
(results from Zfp629 remain inconclusive). Using an antibody against Zipro1, I located
this increase of Zipro1 to the SCs of the denervated CST. I also found Zipro1 expression
in tSCs and an increase of 19% in tSCs of denervated rat muscle. Upregulation of Zipro1
in non-myelinating SCs suggests that Zipro1 may have a role in the activation of tSCs.
Further study is needed in order to clarify the extent of Zipro1's involvement. | |
dc.format.medium | electronic | en |
dc.language.iso | eng | en |
dc.rights | Copyright is held by the author. Presentation of this material on
the Libraries' web site by University Libraries, The University of Texas at Austin was made
possible under a limited license grant from the author who has retained all copyrights in
the works. | en |
dc.subject.lcsh | Sympathetic nervous system | en |
dc.subject.lcsh | Myelination | en |
dc.subject.lcsh | Nerves, Peripheral--Regeneration | en |
dc.subject.lcsh | Zinc-finger proteins | en |
dc.title | Zinc-finger transcription factors and the response of non-myelinating Schwann cells to axonal injury | en |
dc.description.department | Institute for Neuroscience | en |
dc.identifier.oclc | 244393305 | en |
dc.type.genre | Thesis | en |
thesis.degree.department | Neuroscience, Institute for | en |
thesis.degree.discipline | Neuroscience | en |
thesis.degree.grantor | The University of Texas at Austin | en |
thesis.degree.level | Doctoral | en |
thesis.degree.name | Doctor of Philosophy | en |