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dc.creatorSaldanda, Roland J.en
dc.creatorPemberton, Adinen
dc.creatorSchiflett, Patricken
dc.creatorPerutka, Jirien
dc.creatorWhitt, Jacob T.en
dc.creatorEllingotn, Andrewen
dc.creatorLambowitz, Alan M.en
dc.creatorKramer, Ryanen
dc.creatorTaylor, Deborahen
dc.creatorLamkin, Thomas J.en
dc.date.accessioned2014-12-15T17:10:56Zen
dc.date.available2014-12-15T17:10:56Zen
dc.date.issued2013-09-18en
dc.identifier.citationSaldanha, Roland J., Adin Pemberton, Patrick Shiflett, Jiri Perutka, Jacob T. Whitt, Andrew Ellington, Alan M. Lambowitz, Ryan Kramer, Deborah Taylor, and Thomas J. Lamkin. “Rapid Targeted Gene Disruption in Bacillus Anthracis.” BMC Biotechnology 13, no. 1 (September 18, 2013): 72. doi:10.1186/1472-6750-13-72.en
dc.identifier.urihttp://hdl.handle.net/2152/27954en
dc.descriptionRoland J. Saldanha and Adin Pemberton are with UES Inc Dayton, OH USA -- Patrick Shiflett is with the Henry Jackson Foundation Bethesda, MD USA -- Jiri Perutka, Jacob T. Whitt, Andrew Ellington and Alan M. Lamowitz are with the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX -- Ryan Kramer, Deborah Taylor and Thomas J. Lamkin are with the Air Force Research Laboratory, Air Force Research Laboratory, 711th HPW/RHXBC, Molecular Signatures Section, Wright-Patterson AFB, OH USA -- Ryan Kramer is with the Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH USAen
dc.description.abstractBackground: Anthrax is a zoonotic disease recognized to affect herbivores since Biblical times and has the widest range of susceptible host species of any known pathogen. The ease with which the bacterium can be weaponized and its recent deliberate use as an agent of terror, have highlighted the importance of gaining a deeper understanding and effective countermeasures for this important pathogen. High quality sequence data has opened the possibility of systematic dissection of how genes distributed on both the bacterial chromosome and associated plasmids have made it such a successful pathogen. However, low transformation efficiency and relatively few genetic tools for chromosomal manipulation have hampered full interrogation of its genome. Results: Group II introns have been developed into an efficient tool for site-specific gene inactivation in several organisms. We have adapted group II intron targeting technology for application in Bacillus anthracis and generated vectors that permit gene inactivation through group II intron insertion. The vectors developed permit screening for the desired insertion through PCR or direct selection of intron insertions using a selection scheme that activates a kanamycin resistance marker upon successful intron insertion. Conclusions: The design and vector construction described here provides a useful tool for high throughput experimental interrogation of the Bacillus anthracis genome and will benefit efforts to develop improved vaccines and therapeutics.en
dc.description.sponsorshipen
dc.language.isoEnglishen
dc.publisherBMC Biotechnologyen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectanthraxen
dc.subjectbacillus anthracisen
dc.subjectpathogensen
dc.subjectsequence dataen
dc.subjecten
dc.titleRapid targeted gene disruption in Bacillus anthracisen
dc.typeArticleen
dc.description.departmentInstitute for Cellular and Molecular Biologyen
dc.description.catalogingnoteThomas.Lamkin@wpafb.af.milen
dc.identifier.Filename1472-6750-13-72.pdfen
dc.identifier.doidoi:10.1186/1472-6750-13-72en


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