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dc.creatorBerg, A. Hakanen
dc.creatorThomas, Peteren
dc.creatorOlsson, Per-Eriken
dc.date.accessioned2014-12-15T17:10:31Zen
dc.date.available2014-12-15T17:10:31Zen
dc.date.issued2005-11-10en
dc.identifier.citationBerg, A. Håkan, Peter Thomas, and Per-Erik Olsson. “Biochemical Characterization of the Arctic Char (Salvelinus Alpinus) Ovarian Progestin Membrane Receptor.” Reproductive Biology and Endocrinology 3, no. 1 (November 10, 2005): 64. doi:10.1186/1477-7827-3-64.en
dc.identifier.urihttp://hdl.handle.net/2152/27892en
dc.descriptionA. Hakan Berg and Peter Thomas are with The University of Texas at Austin Marine Science Institute, Port Aransas, Texas, USA -- Per-Erik Olsson is witht the Örebro Life Science Center, Department of Natural Sciences, Örebro University, Swedenen
dc.description.abstractMembrane progestin receptors are involved in oocyte maturation in teleosts. However, the maturation-inducing steroid (MIS) does not appear to be conserved among species and several progestins may fulfill this function. So far, complete biochemical characterization has only been performed on a few species. In the present study we have characterized the membrane progestin receptor in Arctic char (Salvelinus alpinus) and show that the 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) receptor also binds several xenobiotics, thus rendering oocyte maturation sensitive to environmental pollutants. We identified a single class of high affinity (Kd, 13.8 ± 1.1 nM), low capacity (Bmax, 1.6 ± 0.6 pmol/g ovary) binding sites by saturation and Scatchard analyses. Receptor binding displayed rapid association and dissociation kinetics typical of steroid membrane receptors, with t1/2 s of less than 1 minute. The 17,20beta-P binding also displayed tissue specificity with high, saturable, and specific 17,20beta-P binding detected in ovaries, heart and gills while no specific binding was observed in muscle, brain or liver. Changes in 17,20beta-P binding during oocyte maturation were consistent with its identity as the oocyte MIS membrane receptor. Incubation of fully-grown ovarian follicles with gonadotropin induced oocyte maturation, which was accompanied by a five-fold increase in 17,20beta-P receptor binding. In addition, competition studies with a variety of steroids revealed that receptor binding is highly specific for 17,20beta-P, the likely maturation-inducing steroid (MIS) in Arctic char. The relative-binding affinities of all the other progestogens and steroids tested were less than 5% of that of 17,20beta-P for the receptor. Several ortho, para derivatives of DDT also showed weak binding affinity for the 17,20beta-P receptor supporting the hypothesis that xenobiotics may bind steroid receptors on the oocyte's surface and might thereby interfere with oocyte growth and maturation.en
dc.description.sponsorshipen
dc.language.isoEnglishen
dc.publisherReproductive Biology and Endocrinologyen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectArctic charen
dc.subjectovarian progestin membrane receptoren
dc.titleBiochemical characterization of the Arctic char (Salvelinus alpinus) ovarian progestin membrane receptoren
dc.typeArticleen
dc.description.departmentMarine Scienceen
dc.description.catalogingnoteper-erik.olsson@nat.oru.seen
dc.identifier.Filename1477-7827-3-64en
dc.identifier.doidoi:10.1186/1477-7827-3-64en


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