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dc.creatorHart, G. Traveren
dc.creatorLee, Insuken
dc.creatorMarcotte, Edward M.en
dc.date.accessioned2014-12-15T17:10:22Zen
dc.date.available2014-12-15T17:10:22Zen
dc.date.issued2007-07-02en
dc.identifier.citationHart, G. Traver, Insuk Lee, and Edward M. Marcotte. “A High-Accuracy Consensus Map of Yeast Protein Complexes Reveals Modular Nature of Gene Essentiality.” BMC Bioinformatics 8, no. 1 (July 2, 2007): 236. doi:10.1186/1471-2105-8-236.en
dc.identifier.urihttp://hdl.handle.net/2152/27870en
dc.descriptionAll author are with the Center for Systems and Synthetic Biology Institute for Cellular and Molecular Biology University of Texas at Austin 2500 Speedway, MBB 3.210 Austin, Texas, 78712, USAen
dc.description.abstractBackground: Identifying all protein complexes in an organism is a major goal of systems biology. In the past 18 months, the results of two genome-scale tandem affinity purification-mass spectrometry (TAP-MS) assays in yeast have been published, along with corresponding complex maps. For most complexes, the published data sets were surprisingly uncorrelated. It is therefore useful to consider the raw data from each study and generate an accurate complex map from a high-confidence data set that integrates the results of these and earlier assays. -- Results: Using an unsupervised probabilistic scoring scheme, we assigned a confidence score to each interaction in the matrix-model interpretation of the large-scale yeast mass-spectrometry data sets. The scoring metric proved more accurate than the filtering schemes used in the original data sets. We then took a high-confidence subset of these interactions and derived a set of complexes using MCL. The complexes show high correlation with existing annotations. Hierarchical organization of some protein complexes is evident from inter-complex interactions. -- Conclusion: We demonstrate that our scoring method can generate an integrated high-confidence subset of observed matrix-model interactions, which we subsequently used to derive an accurate map of yeast complexes. Our results indicate that essentiality is a product of the protein complex rather than the individual protein, and that we have achieved near saturation of the yeast high-abundance, rich-media-expressed "complex-ome."en
dc.description.sponsorshipen
dc.language.isoEnglishen
dc.publisherBMC Bioinformaticsen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectprotein complexesen
dc.subjectsystems biologyen
dc.subjectyeast proteinsen
dc.titleA high-accuracy consensus map of yeast protein complexes reveals modular nature of gene essentialityen
dc.typeArticleen
dc.description.departmentCenter for Systems and Synthetic Biologyen
dc.description.departmentInstitute for Cellular and Molecular Biologyen
dc.description.catalogingnotemarcotte@icmb.utexas.eduen
dc.identifier.Filename1471-2105-8-236en
dc.identifier.doidoi:10.1186/1471-2105-8-236en


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