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dc.creatorPang, Mingxiongen
dc.creatorWoodward, Andrew W.en
dc.creatorAgarwal, Vikramen
dc.creatorGuan, Xueyingen
dc.creatorHa, Misooken
dc.creatorRamachandran, Vanitharanien
dc.creatorChen, Xuemeien
dc.creatorTriplett, Barbara A.en
dc.creatorStelly, David M.en
dc.creatorChen, Z. Jefferyen
dc.date.accessioned2014-12-15T17:10:09Zen
dc.date.available2014-12-15T17:10:09Zen
dc.date.issued2009-11-04en
dc.identifier.citationPang, Mingxiong, Andrew W. Woodward, Vikram Agarwal, Xueying Guan, Misook Ha, Vanitharani Ramachandran, Xuemei Chen, Barbara A. Triplett, David M. Stelly, and Z. Jeffrey Chen. “Genome-Wide Analysis Reveals Rapid and Dynamic Changes in miRNA and siRNA Sequence and Expression during Ovule and Fiber Development in Allotetraploid Cotton (Gossypium Hirsutum L.).” Genome Biology 10, no. 11 (November 4, 2009): R122. doi:10.1186/gb-2009-10-11-r122.en
dc.identifier.urihttp://hdl.handle.net/2152/27837en
dc.description1 Section of Molecular Cell and Developmental Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA -- 2 Institute for Cellular and Molecular Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA -- 3 Center for Computational Biology and Bioinformatics, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USA -- 4 Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA -- 5 USDA-ARS-SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124, USA -- 6 Department of Soil and Crop Sciences, Texas AM University, College Station, TX 77843, USA -- 7 Section of Integrative Biology, The University of Texas at Austin, One University Station, A-4800, Austin, TX 78712, USAen
dc.description.abstractBackground: Cotton fiber development undergoes rapid and dynamic changes in a single cell type, from fiber initiation, elongation, primary and secondary wall biosynthesis, to fiber maturation. Previous studies showed that cotton genes encoding putative MYB transcription factors and phytohormone responsive factors were induced during early stages of ovule and fiber development. Many of these factors are targets of microRNAs (miRNAs) that mediate target gene regulation by mRNA degradation or translational repression. Results: Here we sequenced and analyzed over 4 million small RNAs derived from fiber and non-fiber tissues in cotton. The 24-nucleotide small interfering RNAs (siRNAs) were more abundant and highly enriched in ovules and fiber-bearing ovules relative to leaves. A total of 31 miRNA families, including 27 conserved, 4 novel miRNA families and a candidate-novel miRNA, were identified in at least one of the cotton tissues examined. Among 32 miRNA precursors representing 19 unique miRNA families identified, 7 were previously reported, and 25 new miRNA precursors were found in this study. Sequencing, miRNA microarray, and small RNA blot analyses showed a trend of repression of miRNAs, including novel miRNAs, during ovule and fiber development, which correlated with upregulation of several target genes tested. Moreover, 223 targets of cotton miRNAs were predicted from the expressed sequence tags derived from cotton tissues, including ovules and fibers. The cotton miRNAs examined triggered cleavage in the predicted sites of the putative cotton targets in ovules and fibers. Conclusions: Enrichment of siRNAs in ovules and fibers suggests active small RNA metabolism and chromatin modifications during fiber development, whereas general repression of miRNAs in fibers correlates with upregulation of a dozen validated miRNA targets encoding transcription and phytohormone response factors, including the genes found to be highly expressed in cotton fibers. Rapid and dynamic changes in siRNAs and miRNAs may contribute to ovule and fiber development in allotetraploid cotton.en
dc.description.sponsorshipen
dc.language.isoEnglishen
dc.publisherGenome Biologyen
dc.rightsAdministrative deposit of works to UT Digital Repository: This works author(s) is or was a University faculty member, student or staff member; this article is already available through open access at http://www.biomedcentral.com. The public license is specified as CC-BY: http://creativecommons.org/licenses/by/4.0/. The library makes the deposit as a matter of fair use (for scholarly, educational, and research purposes), and to preserve the work and further secure public access to the works of the University.en
dc.subjectmiRNAen
dc.subjectsiRNAen
dc.subjectallotetraploid cottonen
dc.subjectGossypium hirsutum L.en
dc.titleGenome-wide analysis reveals rapid and dynamic changes in miRNA and siRNA sequence and expression during ovule and fiber development in allotetraploid cotton (Gossypium hirsutum L.)en
dc.typeArticleen
dc.description.departmentInstitute for Cellular and Molecular Biologyen
dc.description.departmentCenter for Computational Biology and Bioinformaticsen
dc.description.catalogingnotezjchen@mail.utexas.eduen
dc.identifier.Filenamegb-2009-10-11-r122en
dc.identifier.doidoi:10.1186/gb-2009-10-11-r122en


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