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dc.contributor.advisorBrown, R. Malcolm (Richard Malcolm), 1939-en
dc.creatorNobles, David Ronalden
dc.date.accessioned2008-08-28T22:56:57Zen
dc.date.available2008-08-28T22:56:57Zen
dc.date.issued2006en
dc.identifierb64850638en
dc.identifier.urihttp://hdl.handle.net/2152/2587en
dc.descriptiontexten
dc.description.abstractMultiple aspects of cellulose biosynthesis in cyanobacteria were investigated: product identification and characterization; function, sequence and phylogenetic analyses of the cellulose synthases; suitability of cyanobacteria for transgenic cellulose synthase expression; and improved tools for the detection and identification of product. Cellulose was positively identified in members of three sections of cyanobacteria. Sequence and phylogenetic analyses established a cyanobacterial origin of vascular plant cellulose synthases. An insertional knockout of a plant-like cellulose synthase caused a loss of heterocyst envelope birefringence, altered the homogeneous polysaccharide layer of the heterocyst, and impaired nitrogen fixation in Nostoc punctiforme. Mutants of Synechococcus leopoliensis strain UTCC 100 expressing genes from the cellulose synthase operon of Acetobacter xylinum secreted extracellular material, which bound Tinopal and CBHI-gold, but could not be positively identified as cellulose. Finally, specific β 1,4-glucan probes for use in fluorescence and electron microscopy were successfully created by fusing the cellulose binding module of Trametes hirsuta to Green Fluorescent Protein (GFP) and Ferritin (FtnA), respectively.
dc.format.mediumelectronicen
dc.language.isoengen
dc.rightsCopyright is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works.en
dc.subject.lcshCyanobacteriaen
dc.subject.lcshCellulose--Synthesisen
dc.titleCellulose in the cyanobacteriaen
dc.description.departmentBiological Sciences, School ofen
dc.identifier.oclc84744364en
dc.type.genreThesisen
thesis.degree.departmentBiological Sciences, School ofen
thesis.degree.disciplinePlant Biologyen
thesis.degree.grantorThe University of Texas at Austinen
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen


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