In vitro polyketide biocatalysis : triketide building-blocks and enzymology
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Date
2013-05
Authors
Harper, Andrew David
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Abstract
Polyketide products are useful compounds to research and industry but can be difficult to access due to their richness in stereogenic centers. Type I polyketide synthases offer unique engineering opportunities to access natural stereocontrol and resultant complex compounds. The development of a controlled in vitro platform based around type I polyketide synthases is described. It has been used to produce a small library of polyketide fragments on an unprecedented and synthetically-relevant scale and explore polyketide synthase enzymology.
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In vitro, Cell-free, Preparative, Polyketide, Polyketide synthase, Ketoreductase, Malonyl-CoA ligase, MatB, Enzymology, Chiral building blocks, Chiral synthons, Diketide, Triketide, Lactone, Ketolactone, Natural products, Secondary metabolism, Secondary metabolite, Modular, Type I polyketide synthase, Biosynthesis, Streptomyces coelicolor, Saccharopolyspora erythraea, Bacillus subtilis, Streptomyces nodosus, Saccharomyces cerevisiae, Streptomyces antibioticus, Streptomyces venezuelae, Saccharopolyspora spinosa, Streptomyces fradiae