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dc.creatorGu, Wanjunen
dc.creatorZhou, Tongen
dc.creatorWilke, Claus O.en
dc.date.accessioned2013-06-28T15:48:55Zen
dc.date.available2013-06-28T15:48:55Zen
dc.date.issued2010-02-05en
dc.identifier.citationGu W, Zhou T, Wilke CO (2010) A Universal Trend of Reduced mRNA Stability near the Translation-Initiation Site in Prokaryotes and Eukaryotes. PLoS Comput Biol 6(2): e1000664. doi:10.1371/journal.pcbi.1000664en
dc.identifier.urihttp://hdl.handle.net/2152/20552en
dc.descriptionWanjun Gu is with Southeast University, Tong Zhou is with UT Austin, Claus O. Wilke is with UT Austin.en
dc.description.abstractRecent studies have suggested that the thermodynamic stability of mRNA secondary structure near the start codon can regulate translation efficiency in Escherichia coli, and that translation is more efficient the less stable the secondary structure. We survey the complete genomes of 340 species for signals of reduced mRNA secondary structure near the start codon. Our analysis includes bacteria, archaea, fungi, plants, insects, fishes, birds, and mammals. We find that nearly all species show evidence for reduced mRNA stability near the start codon. The reduction in stability generally increases with increasing genomic GC content. In prokaryotes, the reduction also increases with decreasing optimal growth temperature. Within genomes, there is variation in the stability among genes, and this variation correlates with gene GC content, codon bias, and gene expression level. For birds and mammals, however, we do not find a genome-wide trend of reduced mRNA stability near the start codon. Yet the most GC rich genes in these organisms do show such a signal. We conclude that reduced stability of the mRNA secondary structure near the start codon is a universal feature of all cellular life. We suggest that the origin of this reduction is selection for efficient recognition of the start codon by initiator-tRNA.en
dc.description.sponsorshipThis work was supported by NIH grant R01 GM088344 to COW and a grant from National Natural Science Foundation of China (No. 30900836) to WG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.rightsAttribution 3.0 United Statesen
dc.rightsCC-BYen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjectAnimal genomicsen
dc.subjectBacterial genomicsen
dc.subjectBird genomicsen
dc.subjectCodonsen
dc.subjectFungal genomicsen
dc.subjectInvertebrate genomicsen
dc.subjectPlant genomicsen
dc.subjectProtein translationen
dc.titleA Universal Trend of Reduced mRNA Stability near the Translation-Initiation Site in Prokaryotes and Eukaryotesen
dc.typeArticleen
dc.description.departmentCellular and Molecular Biologyen
dc.identifier.doi10.1371/journal.pcbi.1000664en


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Attribution 3.0 United States
Except where otherwise noted, this item's license is described as Attribution 3.0 United States