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dc.creatorFernandez, Irinaen
dc.creatorFridley, Krista M.en
dc.creatorArasappan, Dhivyaen
dc.creatorAmbler, Rosalind V.en
dc.creatorTucker, Philip W.en
dc.creatorRoy, Krishnenduen
dc.date.accessioned2013-05-23T16:38:04Zen
dc.date.available2013-05-23T16:38:04Zen
dc.date.issued2012-12-27en
dc.identifier.citationFernandez I, Fridley KM, Arasappan D, Ambler RV, Tucker PW, et al. (2012) Gene Expression Profile and Functionality of ESC-Derived Lin-ckit+Sca-1+ Cells Are Distinct from Lin-ckit+Sca-1+ Cells Isolated from Fetal Liver or Bone Marrow. PLoS ONE 7(12): e51944. doi:10.1371/journal.pone.0051944en
dc.identifier.urihttp://hdl.handle.net/2152/20165en
dc.description.abstractIn vitro bioreactor-based cultures are being extensively investigated for large-scale production of differentiated cells from embryonic stem cells (ESCs). However, it is unclear whether in vitro ESC-derived progenitors have similar gene expression profiles and functionalities as their in vivo counterparts. This is crucial in establishing the validity of ESC-derived cells as replacements for adult-isolated cells for clinical therapies. In this study, we compared the gene expression profiles of Lin-ckit+Sca-1+ (LKS) cells generated in vitro from mouse ESCs using either static or bioreactor-based cultures, with that of native LKS cells isolated from mouse fetal liver (FL) or bone marrow (BM). We found that in vitro-generated LKS cells were more similar to FL- than to BM LKS cells in gene expression. Further, when compared to cells derived from bioreactor cultures, static culture-derived LKS cells showed fewer differentially expressed genes relative to both in vivo LKS populations. Overall, the expression of hematopoietic genes was lower in ESC-derived LKS cells compared to cells from BM and FL, while the levels of non-hematopoietic genes were up-regulated. In order to determine if these molecular profiles correlated with functionality, we evaluated ESC-derived LKS cells for in vitro hematopoietic-differentiation and colony formation (CFU assay). Although static culture-generated cells failed to form any colonies, they did differentiate into CD11c+ and B220+ cells indicating some hematopoietic potential. In contrast, bioreactor-derived LKS cells, when differentiated under the same conditions failed to produce any B220+ or CD11c+ cells and did not form colonies, indicating that these cells are not hematopoietic progenitors. We conclude that in vitro culture conditions significantly affect the transcriptome and functionality of ESC-derived LKS cells and although in vitro differentiated LKS cells were lineage negative and expressed both ckit and Sca-1, these cells, especially those obtained from dynamic cultures, are significantly different from native cells of the same phenotype.en
dc.description.sponsorshipThis work was partially supported through National Institutes of Health grant number EB005026 to KR and PWT. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding received for this study.en
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.rightsAttribution 3.0 United Statesen
dc.rightsCC-BYen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en
dc.subjectBone marrow cellsen
dc.subjectCell differentiationen
dc.subjectEmbryonic stem cellsen
dc.subjectGene expressionen
dc.subjectHematopoiesisen
dc.subjectHematopoietic stem cellsen
dc.subjectStem cell therapyen
dc.subjectSuspensionsen
dc.titleGene Expression Profile and Functionality of ESC-Derived Lin-ckit+Sca-1+ Cells Are Distinct from Lin-ckit+Sca-1+ Cells Isolated from Fetal Liver or Bone Marrowen
dc.typeArticleen
dc.description.departmentBiomedical Engineeringen
dc.identifier.doi10.1371/journal.pone.0051944en


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Attribution 3.0 United States
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