|dc.description.abstract||Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that causes
chronic infections in the lungs of individuals with cystic fibrosis (CF). Like many
Gram-negatives, P. aeruginosa produces outer membrane vesicles (MVs), which
have been shown to package numerous factors including antimicrobial quinolone
molecules, toxins, DNA, antibiotic resistance determinants, and cell-cell signaling
molecules. The mechanism for the formation of MVs has not been fully elucidated.
The Gram-negative outer membrane (OM) contains associated proteins, which
anchor it to the peptidoglycan, and keep the OM stable. We hypothesized that
peptidoglycan-associated outer membrane lipoproteins OprF, OprL, and OprI
contribute to MV formation in P. aeruginosa. In this study, we quantified MVs
harvested from oprF, oprL, and oprI mutants. The MV levels produced by the oprL
and oprI mutants were not significantly different from those produced by the wild
type; however, the oprF mutant showed a three-fold increase in MV production.
These data indicate that OprF plays a significant role in anchoring the outer
membrane to the peptidoglycan and that, in its absence, more MVs are formed.||en