Creation of Vibrio cholerae strains to test the induction of the Feo iron transport system using the recombination-based in vivo expression technology (RIVET) vector
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Cholera is a diarrheal disease caused by the bacterium Vibrio cholerae. Iron is important for survival for this pathogen, both as a regulatory mechanism and as part of enzymes. Although the iron-transport mechanisms of V. cholerae have been studied in great detail, it is still unclear when these mechanisms are active during infection. But, there is now a method to test the expression of these mechanisms. This method is known as the recombination-based in vivo expression technology (RIVET) vector. This is a new technology with which one can look at the induction of transcription of different genes during infection of infant mice. I have inserted the feo gene promoter, which is a ferrous iron uptake mechanism, into this vector. I have then incorporated this vector into two cholera strains, so that we can better understand when this particular iron uptake mechanism is expressed in vivo. These strains will be able to successfully identify when the iron transport genes are expressed during infection.