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Optimizing tyrosine production in yeast via in vivo continuous evolution
Tyrosine is an aromatic amino acid with a wide range of uses. Tyrosine is a precursor for numerous biological compounds ranging from neurotransmitters to hormones. It therefore plays a big role in the pharmaceutical industry. ...
Characterizing gene functions with an overexpression ORF collection in Saccharomyces cerevisiae
My research project aims to discover new eukaryotic gene functions using yeast as a model organism. Yeast (Saccharomyces cerevisiae), are a prototypical eukaryotic model because of its high degree of genetic similarity ...
Characterizing the mechanical parameters of the fission yeast cell wall using atomic force microscopy
While the biological and chemical aspects of the yeast cell have been studied in great depth, much less is known about their physical parameters. The fission yeast cell wall is a dynamic structure that is subject to ...
Definition of the yeast transcriptome using next-generation RNA sequencing
RNA sequencing (RNA-seq) is a high-throughput method by which the sequence of each RNA molecule in an organism can be determined. Because of its high-throughput nature and high accuracy, RNA-seq allows for the study of ...
Optimization of chromatin immunoprecipitation
Transcription factors play a vital role in controlling cell growth, and locations of their binding sites at various points of the cell cycle can provide clues about malfunctions in eukaryotic growth, such as cancer. Our ...