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Alternative Computational Protocols for Supercharging Protein Surfaces for Reversible Unfolding and Retention of Stability

Der, Bryan S.; Kluwe, Christien; Miklos, Aleksandr E.; Jacak, Ron; Lyskov, Sergey; Gray, Jeffery J. -Johns Hopkins University, Baltimore, Maryland,; Georgiou, George; Ellington, Andrew D.; Kuhlman, Brian - University of North Carolina at Chapel Hill (PLOS One, 2013-05-31)

Reengineering protein surfaces to exhibit high net charge, referred to as “supercharging”, can improve reversibility of unfolding by preventing aggregation of partially unfolded states. Incorporation of charged side chains ...