Cloning, expression, and purification of Burkholderia protein targets for diagnostic and vaccine development

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Cloning, expression, and purification of Burkholderia protein targets for diagnostic and vaccine development

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dc.contributor.advisor Brown, Katherine A.
dc.contributor.advisor Kitto, George B.
dc.creator McCaul, Kate Christina
dc.date.accessioned 2012-07-18T21:08:02Z
dc.date.available 2012-07-18T21:08:02Z
dc.date.created 2012-05
dc.date.issued 2012-07-18
dc.date.submitted May 2012
dc.identifier.uri http://hdl.handle.net/2152/ETD-UT-2012-05-5601
dc.description.abstract Burkholderia pseudomallei and Burkholderia mallei cause the diseases melioidosis and glanders, respectively. These diseases are endemic mainly in southeastern Asia and northern Australia, but they also pose a bioterrorism threat in the developed world. These diseases have high mortality, partially due to the lack of vaccines and rapid, accurate diagnostic assays. The work discussed here represents a part of a larger project to develop a dependable diagnostic assay for use in both developing endemic areas and the developed world, as well as a subunit vaccine to protect against disease. In this study, several proteins from B. pseudomallei, B. mallei, and the closely related but less virulent B. thailandensis have been cloned, expressed and purified in order to develop highly sensitive and specific diagnostic reagents for the detection of B. pseudomallei and B. mallei in infected patient samples. Protein targets expressed in this study were also used in subunit vaccine development for melioidosis and glanders.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subject Burkholderia pseudomallei
dc.subject Burkholderia mallei
dc.subject Diagnostics
dc.subject Vaccine
dc.subject Protein
dc.subject Expression
dc.subject Cloning
dc.subject Purification
dc.title Cloning, expression, and purification of Burkholderia protein targets for diagnostic and vaccine development
dc.date.updated 2012-07-18T21:08:49Z
dc.identifier.slug 2152/ETD-UT-2012-05-5601
dc.description.department Cellular and Molecular Biology
dc.type.genre thesis
dc.type.material text
thesis.degree.department Cellular and Molecular Biology
thesis.degree.discipline Cell and Molecular Biology
thesis.degree.grantor University of Texas at Austin
thesis.degree.level Masters
thesis.degree.name Master of Arts

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