The HOPS complex and Vps33 in Dictyostelium discoideum

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The HOPS complex and Vps33 in Dictyostelium discoideum

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dc.contributor.advisor Arturo De Lozanne
dc.creator Caffarelli, Nicolas
dc.date.accessioned 2011-09-02T15:26:53Z
dc.date.available 2011-09-02T15:26:53Z
dc.date.created 2009
dc.date.issued 2011-09-02
dc.identifier.uri http://hdl.handle.net/2152/13384
dc.description.abstract In order for cells to eat, they must detect food at their cell surface and pull it in to form a vesicle known as an endosome. This endosome then becomes acidified to become a lysosome and eventually becomes neutral again, as a postlysosome, so that waste can be expelled from the cell. Collectively, this is referred to as the endocytic pathway. Several proteins are involved in this process – Rabs are known to mediate specificity of fusing vesicles, and SNARES catalyze the actual vesicle fusion. In this study we look at the protein Vps33, a subunit of the HOPS complex. The HOPS complex is known to interact with Rabs and SNARES, and we are interested as to where it acts within the cell. By tagging Vps33 with Green Fluorescent Protein (GFP), we can visualize its localization under the microscope. We observe here that Vps33 localizes primarily to the cytoplasm, with sparse localization to intracellular vesicles.
dc.language.iso eng
dc.subject College of Natural Sciences
dc.subject endocytic pathway
dc.subject proteins
dc.subject Vps33
dc.subject HOPS complex
dc.subject Rabs
dc.subject SNARES
dc.title The HOPS complex and Vps33 in Dictyostelium discoideum
dc.type Thesis
dc.description.department Biological Sciences, School of

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