Stability of C-Reactive Protein in saliva using an ELISA test

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Stability of C-Reactive Protein in saliva using an ELISA test

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Title: Stability of C-Reactive Protein in saliva using an ELISA test
Author: Peddaiahgari, Rajesh
Abstract: This project was done in order to measure the stability of proteins in general; in particular C-Reactive Protein (CRP) which is very important in cardiovascular studies. Current methods of handling CRP is to freeze the sample at -80°C for long term use, or -20°C for medium term use, or 4°C for short term use. This experiment was done in order to measure how much CRP will degrade after a certain time with different matrices. From this project, no conclusive data was gathered to give a certain approximation on the stability of CRP. The data that was gathered did tell us that CRP is most stable with PBS with 0.5% Albumin (PBSA) than saliva, which is more stable than in nanopure water. Furthermore, the data showed that samples kept at -20°C were more stable than those kept at 4°C.
Department: Biochemistry
Subject: College of Natural Sciences proteins C-Reactive Protein saliva ELISA
URI: http://hdl.handle.net/2152/13367
Date: 2008

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