Stability of C-Reactive Protein in saliva using an ELISA test
| Title: | Stability of C-Reactive Protein in saliva using an ELISA test |
| Author: | Peddaiahgari, Rajesh |
| Abstract: | This project was done in order to measure the stability of proteins in general; in particular C-Reactive Protein (CRP) which is very important in cardiovascular studies. Current methods of handling CRP is to freeze the sample at -80°C for long term use, or -20°C for medium term use, or 4°C for short term use. This experiment was done in order to measure how much CRP will degrade after a certain time with different matrices. From this project, no conclusive data was gathered to give a certain approximation on the stability of CRP. The data that was gathered did tell us that CRP is most stable with PBS with 0.5% Albumin (PBSA) than saliva, which is more stable than in nanopure water. Furthermore, the data showed that samples kept at -20°C were more stable than those kept at 4°C. |
| Department: | Biochemistry |
| Subject: |
College of Natural Sciences
proteins C-Reactive Protein saliva ELISA |
| URI: | http://hdl.handle.net/2152/13367 |
| Date: | 2008 |
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